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cerebrospinal fluid  (Tocris)
94
Tocris cerebrospinal fluid
A) The gelation time of GliaTrap was determined using a tube inversion method at 37°C. B) The swelling ratio of GliaTrap was studied in an artificial <t>cerebrospinal</t> fluid. GliaTrap shows low (up to 5%) swelling. C) In vitro CXCL12 release kinetics from GliaTrap show sustained release of CXCL12 over 15 consecutive days. The cumulative amount of CXCL12 was measured by ELISA. D & E) Cytokine arrays using brain lysates from control mice (DPBS), mice with implanted HA/Col II hydrogel only, mice with implanted HA/Col II hydrogel + Blank liposomes and mice with implanted GliaTrap (HA/Col II hydrogel + CXCL12-loaded Liposomes). Quantification of the amount of each cytokine of each group was performed by calculating [signal intensity of a dot for each cytokine] / [signal intensity of reference dot] and comparison were performed with Anova. GliaTrap does not have any effect on pro-inflammatory cytokine levels in the brain.
Cerebrospinal Fluid, supplied by Tocris, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/artificial+csf+samples/bio_rxiv__2023__04__12__536581-194-7-10?v=Tocris
Average 94 stars, based on 1 article reviews
cerebrospinal fluid - by Bioz Stars, 2026-07
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cerebrospinal samples fluid acsf  (Tocris)
96
Tocris cerebrospinal samples fluid acsf
A) The gelation time of GliaTrap was determined using a tube inversion method at 37°C. B) The swelling ratio of GliaTrap was studied in an artificial <t>cerebrospinal</t> fluid. GliaTrap shows low (up to 5%) swelling. C) In vitro CXCL12 release kinetics from GliaTrap show sustained release of CXCL12 over 15 consecutive days. The cumulative amount of CXCL12 was measured by ELISA. D & E) Cytokine arrays using brain lysates from control mice (DPBS), mice with implanted HA/Col II hydrogel only, mice with implanted HA/Col II hydrogel + Blank liposomes and mice with implanted GliaTrap (HA/Col II hydrogel + CXCL12-loaded Liposomes). Quantification of the amount of each cytokine of each group was performed by calculating [signal intensity of a dot for each cytokine] / [signal intensity of reference dot] and comparison were performed with Anova. GliaTrap does not have any effect on pro-inflammatory cytokine levels in the brain.
Cerebrospinal Samples Fluid Acsf, supplied by Tocris, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/artificial+csf+samples/pm29519430-63-2-15?v=Tocris
Average 96 stars, based on 1 article reviews
cerebrospinal samples fluid acsf - by Bioz Stars, 2026-07
96/100 stars
  Buy from Supplier

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A) The gelation time of GliaTrap was determined using a tube inversion method at 37°C. B) The swelling ratio of GliaTrap was studied in an artificial cerebrospinal fluid. GliaTrap shows low (up to 5%) swelling. C) In vitro CXCL12 release kinetics from GliaTrap show sustained release of CXCL12 over 15 consecutive days. The cumulative amount of CXCL12 was measured by ELISA. D & E) Cytokine arrays using brain lysates from control mice (DPBS), mice with implanted HA/Col II hydrogel only, mice with implanted HA/Col II hydrogel + Blank liposomes and mice with implanted GliaTrap (HA/Col II hydrogel + CXCL12-loaded Liposomes). Quantification of the amount of each cytokine of each group was performed by calculating [signal intensity of a dot for each cytokine] / [signal intensity of reference dot] and comparison were performed with Anova. GliaTrap does not have any effect on pro-inflammatory cytokine levels in the brain.

Journal: bioRxiv

Article Title: Engineering GliaTrap: a biodegradable non-swelling hydrogel with tuned release of CXCL12 to attract migrating glioblastoma cells

doi: 10.1101/2023.04.12.536581

Figure Lengend Snippet: A) The gelation time of GliaTrap was determined using a tube inversion method at 37°C. B) The swelling ratio of GliaTrap was studied in an artificial cerebrospinal fluid. GliaTrap shows low (up to 5%) swelling. C) In vitro CXCL12 release kinetics from GliaTrap show sustained release of CXCL12 over 15 consecutive days. The cumulative amount of CXCL12 was measured by ELISA. D & E) Cytokine arrays using brain lysates from control mice (DPBS), mice with implanted HA/Col II hydrogel only, mice with implanted HA/Col II hydrogel + Blank liposomes and mice with implanted GliaTrap (HA/Col II hydrogel + CXCL12-loaded Liposomes). Quantification of the amount of each cytokine of each group was performed by calculating [signal intensity of a dot for each cytokine] / [signal intensity of reference dot] and comparison were performed with Anova. GliaTrap does not have any effect on pro-inflammatory cytokine levels in the brain.

Article Snippet: The sample was incubated in an artificial cerebrospinal fluid (aCSF, Tocris Bioscience) at 37°C to allow free swelling for 48 hours.

Techniques: In Vitro, Enzyme-linked Immunosorbent Assay, Control, Liposomes, Comparison